High and fluctuating levels of ovarian hormones induce an anxiogenic effect, which can be modulated under stress conditions: Evidence from an assisted reproductive rodent model.

Elevated levels of endogenous ovarian hormones are conditions commonly experienced by women undergoing assisted reproductive technologies (ART). Additionally, infertility-associated stress and treatment routines are factors that together may have a highly negative impact on female emotionality, which can be aggravated when several cycles of ART are needed to attempt pregnancy. This study aimed to investigate the effect of high and fluctuating levels of gonadal hormones induced by repeated ovarian stimulation on the stress response in rodents. To mimic the context of ART, female rats were exposed to an unpredictable chronic mild stress (UCMS) paradigm for four weeks. During this time, three cycles of ovarian stimulation (superovulation) (150 IU/Kg of PMSG and 75 IU/Kg of hCG) were applied, with intervals of two estrous cycles between them. The rats were distributed into four groups: Repeated Superovulation/UCMS; Repeated Superovulation/No Stress; Saline/UCMS; and Saline/No Stress. Anxiety-like and depressive-like behaviors were evaluated in a light-dark transition box and by splash test, respectively. Corticosterone, estradiol, progesterone, and biometric parameters were assessed. Data were analyzed using a two-way Generalized Linear Model (GzLM). Our results showed that repeated ovarian stimulation exerts by itself an expressive anxiogenic effect. Surprisingly, when high and fluctuating levels of ovarian hormones were combined with chronic stress, anxiety-like behavior was no longer observed, and a depressive-like state was not detected. Our findings suggest that females subjected to emotional overload induced by repeated ovarian stimulation and chronic stress seem to trigger the elaboration of adaptive coping strategies.

Produção científica sobre fluorose em regiões endêmicas: estudo bibliométrico de Anais das Reuniões da Sociedade Brasileira de Pesquisa Odontológica / Scientific production on fluorosis in endemic regions: bibliometric study of Meeting Annals of the Brazilian Society of Dental Research

Objetivo: Identificar os resumos publicados nas Reuniões Anuais da Sociedade Brasileira de Pesquisa Odontológica sobre fluorose em regiões endêmicas e verificar a contribuição da Ciência Brasileira para o assunto. Métodos: Este estudo descritivo foi realizado com dados secundários do período entre 2005 e 2020; os estudos elegíveis foram identificados a partir dos descritores presentes no título e/ou resumo: flúor, fluorose, fluorose dental, fluorose dentária, fluoretação, intoxicação por flúor. Critérios de inclusão: estudos em regiões declaradas como de fluorose endêmica ou que citassem o teor de flúor acima de 0,8 mg F/L. Foram excluídos estudos que não declarassem estudar fluorose em regiões endêmicas ou não citassem o teor de flúor na água. Os dados foram coletados por dois examinadores de modo independente e a distribuição de frequências dos dados foi realizada (Microsoft Excel para Mac v.16.16.12). Resultados: Observou-se que de 41.953 resumos publicados durante esses 15 anos, 1190 (2,83%) foram elegíveis; 426 (1,01%) abordaram especificamente o tema fluorose e24 (0,06%) a fluorose em regiões endêmicas. Os assuntos abordados envolveram tratamento (n = 4; 14,81%); desenvolvimento de programas de promoção de saúde bucal (n = 2; 7,69%);avaliação da potabilidade da água (n = 2; 7,41%); implementação de sistemas de desfluoretação (n = 2; 7,41%); prevalência e severidade (n = 2; 7,41%); distribuição da fluorose na dentição (n = 1, 3,70%); impacto na qualidade de vida (n = 2; 7,41%); percepçãoestética(n = 5; 18,52%); uso e ingestão de flúor (n = 3; 11,11%); fluorose óssea (n = 2; 7,41%); análise da possibilidade de alteração no padrão cognitivo (n = 1; 3,70%), nas funções motoras e no equilíbrio (n=1; 3,70%).Conclusão: Diferentes aspectos relacionados à fluorose em região endêmica foram abordados. A Ciência Brasileira tem se dedicado ao tema ao longo dos anos. Entretanto, há um número restrito de estudos, em especial sobre a relação dose-reposta.

Aim: Identify the abstracts published at the Brazilian Society for Dental Research's Annual Meetings regarding Fluorosis in endemic regions, as well as verify the contribution of Brazilian science to the subject. Methods:This descriptive study was conducted using secondary data from the period between 2005 and 2020; the eligible studies were identified from the descriptors present in the title and/or abstract, as follows: fluoride, fluorosis, dental fluorosis, dental fluorosis, fluoridation, fluoride poisoning. Inclusion criteria: studies in regions with endemic fluorosis were included. Studies that did not address fluorosis in endemic regions were excluded. Data were collected by two independent examiners and the distribution frequency of the data was performed (Microsoft Excel for Mac v.16.16.12). Results: It was observed that of the 41,953 abstracts published during these 15 years, 1,190 (2.83%) were eligible; 426 (1.01%) specifically addressed the theme of fluorosis, and of these, 24 (0.06%) reported on fluorosis in endemic regions. The subjects addressed involved treatment (n = 4; 14.41%), the development of oral health promotion programs (n = 2; 7.41%), the evaluation of water potability (n = 2; 7.41%), the implementation of defluoridation systems (n = 2; 7.41%), prevalence and severity (n = 2; 7.41%), the distribution of fluorosis in dentition (n = 1, 3.70%), impacts on the quality of life (n = 2; 7.41%), esthetic perceptions (n = 4; 18.52%), fluoride use and ingestion (n = 3; 11.11%), bone fluorosis (n = 2; 7.41%), the analysis of alterations in the cognitive pattern (n = 1; 3.70%) and change in motor functions and balance (n=1; 3,70%).Conclusion: Different aspects related to fluorosis in an endemic region were addressed. Brazilian Science has been devoted to the theme over the years; however, there have been a limited number of studies published in the literature concerning the dose-response ratio.

Comparison of the prognostic value of myocardial perfusion imaging using a CZT-SPECT camera with a conventional anger camera.

BACKGROUND: Recent studies have shown that myocardial perfusion imaging (MPI) in cadmium-zinc-telluride (CZT) cameras allow faster exams with less radiation dose but there are little data comparing its prognosis information with that of dedicated cardiac Na-I SPECT cameras OBJECTIVE: The objective of this study is to compare the prognostic value of MPI using an ultrafast protocol with low radiation dose in a CZT-SPECT and a traditional one. METHODS: Group 1 was submitted to a two-day MIBI protocol in a conventional camera, and group 2 was submitted to a 1-day MIBI protocol in CZT camera. MPI were classified as normal or abnormal, and perfusion scores were calculated. Propensity score matching methods were performed RESULTS: 3554 patients were followed during 33±8 months. Groups 1 and 2 had similar distribution of age, gender, body mass index, risk factors, previous revascularization, and use of pharmacological stress. Group 1 had more abnormal scans, higher scores than group 2. Annualized hard events rate was higher in group 1 with normal scans but frequency of revascularization was similar to normal group 2. Patients with abnormal scans had similar event rates in both groups CONCLUSION: New protocol of MPI in CZT-SPECT showed similar prognostic results to those obtained in dedicated cardiac Na-I SPECT camera, with lower prevalence of hard events in patients with normal scan.